c18 analytical column Search Results


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MACHEREY NAGEL nucleosil c 18 column
Attempts of stepwise synthesis of [Met(O)‐432]‐IgG1‐Fc 398–450 ( 1 ). A, Assembly attempt on polystyrene‐divinylbenzene 2‐chlorotrityl resin preloaded with glycine (0.63 mmol/g). The RP‐HPLC profiles of the crude peptide acids with free N ‐terminus at cycle 22 (till Cys‐429) and 32 (till Ser‐419, 1a TRT ) were obtained by using method A. B, Assembly attempt on the polar and low‐loaded resin NovaSyn TGT (0.2 mmol/g Fmoc‐glycine) by using three pseudoproline dipeptides (in red). The RP‐HPLC profile of the Fmoc‐protected crude peptide acid at cycle 44 (till Ser‐407, 1a TGT+ψ ) was obtained by using method C with the <t>Nucleosil</t> C‐18 column (MALDI‐TOF‐MS peaks for M + H + and [M + 2H + ]/2. M calc. for C 242 H 352 N 62 O 69 S 2 : 5298.01 Da)
Nucleosil C 18 Column, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MACHEREY NAGEL nucleodur c18 gravity
Attempts of stepwise synthesis of [Met(O)‐432]‐IgG1‐Fc 398–450 ( 1 ). A, Assembly attempt on polystyrene‐divinylbenzene 2‐chlorotrityl resin preloaded with glycine (0.63 mmol/g). The RP‐HPLC profiles of the crude peptide acids with free N ‐terminus at cycle 22 (till Cys‐429) and 32 (till Ser‐419, 1a TRT ) were obtained by using method A. B, Assembly attempt on the polar and low‐loaded resin NovaSyn TGT (0.2 mmol/g Fmoc‐glycine) by using three pseudoproline dipeptides (in red). The RP‐HPLC profile of the Fmoc‐protected crude peptide acid at cycle 44 (till Ser‐407, 1a TGT+ψ ) was obtained by using method C with the <t>Nucleosil</t> C‐18 column (MALDI‐TOF‐MS peaks for M + H + and [M + 2H + ]/2. M calc. for C 242 H 352 N 62 O 69 S 2 : 5298.01 Da)
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MACHEREY NAGEL macherey nagel nucleodur c18 htec columns
Attempts of stepwise synthesis of [Met(O)‐432]‐IgG1‐Fc 398–450 ( 1 ). A, Assembly attempt on polystyrene‐divinylbenzene 2‐chlorotrityl resin preloaded with glycine (0.63 mmol/g). The RP‐HPLC profiles of the crude peptide acids with free N ‐terminus at cycle 22 (till Cys‐429) and 32 (till Ser‐419, 1a TRT ) were obtained by using method A. B, Assembly attempt on the polar and low‐loaded resin NovaSyn TGT (0.2 mmol/g Fmoc‐glycine) by using three pseudoproline dipeptides (in red). The RP‐HPLC profile of the Fmoc‐protected crude peptide acid at cycle 44 (till Ser‐407, 1a TGT+ψ ) was obtained by using method C with the <t>Nucleosil</t> C‐18 column (MALDI‐TOF‐MS peaks for M + H + and [M + 2H + ]/2. M calc. for C 242 H 352 N 62 O 69 S 2 : 5298.01 Da)
Macherey Nagel Nucleodur C18 Htec Columns, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MACHEREY NAGEL nucleosil 100
Attempts of stepwise synthesis of [Met(O)‐432]‐IgG1‐Fc 398–450 ( 1 ). A, Assembly attempt on polystyrene‐divinylbenzene 2‐chlorotrityl resin preloaded with glycine (0.63 mmol/g). The RP‐HPLC profiles of the crude peptide acids with free N ‐terminus at cycle 22 (till Cys‐429) and 32 (till Ser‐419, 1a TRT ) were obtained by using method A. B, Assembly attempt on the polar and low‐loaded resin NovaSyn TGT (0.2 mmol/g Fmoc‐glycine) by using three pseudoproline dipeptides (in red). The RP‐HPLC profile of the Fmoc‐protected crude peptide acid at cycle 44 (till Ser‐407, 1a TGT+ψ ) was obtained by using method C with the <t>Nucleosil</t> C‐18 column (MALDI‐TOF‐MS peaks for M + H + and [M + 2H + ]/2. M calc. for C 242 H 352 N 62 O 69 S 2 : 5298.01 Da)
Nucleosil 100, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Attempts of stepwise synthesis of [Met(O)‐432]‐IgG1‐Fc 398–450 ( 1 ). A, Assembly attempt on polystyrene‐divinylbenzene 2‐chlorotrityl resin preloaded with glycine (0.63 mmol/g). The RP‐HPLC profiles of the crude peptide acids with free N ‐terminus at cycle 22 (till Cys‐429) and 32 (till Ser‐419, 1a TRT ) were obtained by using method A. B, Assembly attempt on the polar and low‐loaded resin NovaSyn TGT (0.2 mmol/g Fmoc‐glycine) by using three pseudoproline dipeptides (in red). The RP‐HPLC profile of the Fmoc‐protected crude peptide acid at cycle 44 (till Ser‐407, 1a TGT+ψ ) was obtained by using method C with the Nucleosil C‐18 column (MALDI‐TOF‐MS peaks for M + H + and [M + 2H + ]/2. M calc. for C 242 H 352 N 62 O 69 S 2 : 5298.01 Da)

Journal: Journal of Peptide Science

Article Title: An explorative study towards the chemical synthesis of the immunoglobulin G1 Fc CH3 domain

doi: 10.1002/psc.3126

Figure Lengend Snippet: Attempts of stepwise synthesis of [Met(O)‐432]‐IgG1‐Fc 398–450 ( 1 ). A, Assembly attempt on polystyrene‐divinylbenzene 2‐chlorotrityl resin preloaded with glycine (0.63 mmol/g). The RP‐HPLC profiles of the crude peptide acids with free N ‐terminus at cycle 22 (till Cys‐429) and 32 (till Ser‐419, 1a TRT ) were obtained by using method A. B, Assembly attempt on the polar and low‐loaded resin NovaSyn TGT (0.2 mmol/g Fmoc‐glycine) by using three pseudoproline dipeptides (in red). The RP‐HPLC profile of the Fmoc‐protected crude peptide acid at cycle 44 (till Ser‐407, 1a TGT+ψ ) was obtained by using method C with the Nucleosil C‐18 column (MALDI‐TOF‐MS peaks for M + H + and [M + 2H + ]/2. M calc. for C 242 H 352 N 62 O 69 S 2 : 5298.01 Da)

Article Snippet: Analytical RP‐HPLC was performed using a Thermo Fisher Scientific Dionex UltiMate 3000 UHPLC system (Germering, Germany) and either a Syncronics C‐18 column (100 Å, 5 μm, 250 × 4.6 mm, Thermo Fisher Scientific) at a flow rate of 1.5 mL/min or a Nucleosil C‐18 column (100 Å, 5 μm, 250 × 4 mm, Macherey‐Nagel, Düren, Germany) at a flow rate of 1 mL/min.

Techniques: